Document 0783
 DOCN  M94B0783
 TI    The endothelium in HIV-associated Kaposi's sarcoma.
 DT    9412
 AU    Corbeil J; Univ. of New South Wales, Australia
 SO    Diss Abstr Int [B]; 54(6):3004 1993. Unique Identifier : AIDSLINE
       ICDB/94605793
 AB    The aim of this project was to culture Kaposi's sarcoma-derived (KS)
       cells in order to investigate its etiology and attempt to elucidate the
       role of the vascular endothelial cell (EC) in this pathological process.
       The establishment of five cell cultures derived from pleural or
       peritoneal fluid of HIV-infected individuals with KS, and one without,
       are described. These cells originate from vascular endothelium since
       specific staining for EC markers and the secretion of endothelin, a
       vascular EC product were demonstrated. The KS-derived cells secreted
       large amounts of cytokines (GM-CSF, TNF-alpha, IL-1 beta and especially
       IL-6). Conditioned media from the KS-derived cells prompted normal
       capillary EC to proliferate. The KS-derived cells synthesized fibroblast
       growth factor (FGF)-like molecules in amounts sufficient to induce the
       proliferation of normal EC and fibroblasts. These data support the
       existence of a paracrine pathway of EC proliferation in KS and suggest
       that KS-derived cells could sustain their own growth via an autocrine
       mechanism. Furthermore, we studied the effect of in vitro infection of
       vascular EC with HIV-1. Low levels of viral antigen ranging from 30 to
       225 pg/mL were demonstrated and the expression was transient. Immunogold
       electron microscopy using monoclonal antibodies specific for p18, p24
       and gp41 showed the presence of HIV. Levels of IL-6 were increased at
       day 3 of the infection compared to EC inoculated with Hut78 supernatant
       or DEAE-dextran treated EC. These results are the first description that
       EC can be productively infected in vitro and that viral infection of EC
       induces the production of IL-6. The relationship between the
       HIV-infection of EC and KS cells secretory capability could possibly
       lead us to a clearer picture of the neopathogenesis of KS. (Full text
       NOT AVAILABLE FROM UNIVERSITY MICROFILMS INT'L.)
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Cell Division
       Culture Media, Conditioned  Cytokines/SECRETION  Endothelium,
       Vascular/MICROBIOLOGY/*PATHOLOGY/SECRETION  HIV-1/ISOLATION & PURIF
       Microscopy, Electron  Sarcoma, Kaposi's/*ETIOLOGY/PATHOLOGY  Tumor
       Cells, Cultured  THESIS

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